| 货号 | 31404 |
| 别名 | AIM, DNMT, MCMT, CXXC9, HSN1E, ADCADN |
| 使用方法 | Recombinant DNMT1 protein is suitable for use in enzyme kinetics, inhibitor screening, and selectivity profiling. DNMT Activity Assay Conditions: Assay was performed using DNMT Activity / Inhibition Assay, Active Motif Catalog No. 55006, for 1 hour at 37°C. |
| 供应商 | Active Motif |
| 背景 | DNMT1 (DNA (Cytosine-5-)-Methyltransferase 1) proteins are involved in DNA methylation in which a methyl group is added to a cytosine residue on DNA, commonly at the C5 position of a CpG dinucleotide. Three families of DNMTs have been identified: DNMT1, DNMT2, and DNMT3 (comprised of DNMT3A and DNMT3B). DNMT1 is the most abundant DNMT in somatic cells and shows a preference for methylating hemi-methylated DNA. It is considered to be a maintenance DNA methyltransferase that is important in the maintenance of specific patterns of methylation throughout cellular divisions. Methylation of mammalian DNA has long been recognized to play a major role in a number of cellular functions such as embryonic development, genetic imprinting, X chromosome inactivation and the control of gene expression. DNA methylation is generally associated with transcriptional repression. |
| 运输条件 | Dry Ice |
| 存放说明 | Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 外观 | Full length recombinant DNMT1 protein is supplied at a concentration of 0.55 µg/µl in 25 mM Tris pH 8.0, 500 mM NaCl, 5% Glycerol, 0.04% Triton X-100, 1mM DTT and 0.2 mg/ml 3 × Flag peptide. |
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Recombinant DNMT1 protein gel. Recombinant DNMT1 protein was run on an 8% SDS-PAGE gel and stained with Coomassie blue. | |
Recombinant DNMT1 protein activity assay Recombinant DNMT1 protein activity was measured using DNMT Activity / Inhibition Assay, Active Motif Catalog No. 55006, for 1 hour at 37°C |
