| 货号 | 31510 |
| 别名 | PFM6, MSBP3, ZNF899, MEISETZ |
| 使用方法 | Recombinant PRDM9 is suitable for use in enzyme kinetics, inhibitor screening, and selectivity profiling. Specific Activity: H3K4me3 methyltransferase HMT Assay Conditions: 50 mM TrisCl, pH 8.5, 30 mM NaCl, 5% glycerol, 2 mM DTT, 5, 25, and 50 μM H3 (1-24) peptide, 100 nM Recombinant PRMT5 protein at room temperature. |
| 供应商 | Active Motif |
| 背景 | PRDM9 (PR Domain Containing 9) is a Histone methyltransferase that specifically trimethylates Lys-4 of histone H3 during meiotic prophase and is essential for proper meiotic progression. Does not have the ability to mono- and dimethylate Lys-4 of histone H3. H3 Lys-4 methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in the transcriptional activation of genes during early meiotic prophase (By similarity). |
| 运输条件 | Dry Ice |
| 存放说明 | Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 外观 | Recombinant PRDM9 protein was expressed in E.coli and supplied at a concentration of 1.2 μg/μl in 20 mM Tris pH 7.5, 300 mM NaCl, 0.5 mM TCEP, 5 % glycerol, 1 μM ZnCl2. |
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Recombinant PRDM9 protein gel. PRDM9 protein was run on a 12% SDS-PAGE gel and stained with Coomassie blue. | |
Recombinant PRDM9 protein activity assay. PRDM9 protein was used in an HMT assay to determine enzyme acticvity. Activity was detected by MALDI-TOF. |
