| 货号 | 31507 |
| 别名 | Lysine Demethylase 1A, Flavin-Containing Amine Oxidase Domain-Containing Protein 2, Lysine-Specific Histone Demethylase 1 |
| 使用方法 | Recombinant LSD1 / KDM1A is suitable for use in histone demethylation assays. A recommended starting point for the demethylation assay is 20-100 ng of recombinant protein per reaction. |
| 供应商 | Active Motif |
| 背景 | LSD1 (Lysine-specific demethylase 1, also designated KDM1A) was identified as a subunit of different complexes (such as CTBP, CoREST, NuRD or BRAF35). LSD1 is involved in transcriptional repression of genes through the demethylation of Histone H3 monomethyl Lys4 and Histone H3 dimethyl Lys4, a methylation site associated with transcriptional activation. LSD1 is also involved in transcriptional activation through the demethylation of Histone H3 monomethyl Lys9 and Histone H3 dimethyl Lys9, a modification often associated with transcriptional repression. LSD1 is also able to demethylate Lys370me1 and Lys370me2 in the regulatory domain of the tumor suppressor p53. LSD1 is a flavin-dependent amine oxidase. |
| 运输条件 | Dry Ice |
| 存放说明 | Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 外观 | Recombinant LSD1 / KDM1A protein expressed in E. coli cells supplied at a concentration of 0.32 µg/µl in 20 mM Na2HPO4/ NaH2PO4, pH 7.4, 300 mM NaCl, 16 mM glutathione, 1 mM EDTA, 0.05%Tween-20, and 20% glycerol. |
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Recombinant LSD1 / KDM1A protein gel. LSD1 / KDM1A run on a 4-20% SDS-PAGE gel and stained with Coomassie blue. | |
Recombinant LSD1 / KDM1A protein activity assay. Recombinant LSD1 / KDM1A activity measured using a fluorescent demethylase assay. |
