| 货号 | 31502 |
| 别名 | Monoamine Oxidase A, MAO-A |
| 使用方法 | Recombinant MAOA is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. Monoamine Oxidase Assay: 40 uM substrate was incubated with MAOA protein in reaction buffer for 1 hour at room temperature, then the product was incubated with Fluorescence for 0.5 hour at temperature. The activity of MAOA was detected by Promega MAO-GloTMAssay. |
| 供应商 | Active Motif |
| 背景 | MAOA (Monoamine Oxidase A) is one of two neighboring mitochondrial enzyme family members which catalyze the oxidative deamination of amines, such as dopamine, norepinephrine, and serotonin. The other member is MAOB. Mutation of MAOA results in Brunner syndrome and maoa-related behavior disorders. MAOA has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. MAOA preferentially oxidizes biogenic amines such as 5-hydroxytryptamine (5-HT), norepinephrine and epinephrine. |
| 运输条件 | Dry Ice |
| 存放说明 | Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 外观 | Full length recombinant MAOA protein was expressed in Sf9 cells and supplied at a concentration of 0.35 μg/μl in 25 mM Hepes pH 7.5, 300 mM NaCl, 5% Glycerol, 0.04% Triton X-100, 0.2 mM TCEP, 0.2 mg/ml 3× Flag peptide. |
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Recombinant MAOA protein gel. MAOA protein was run on an 8% SDS-PAGE gel and stained with Coomassie blue. | |
Recombinant MAOA activity assay. 40 uM substrate was incubated with MAOA protein in reaction buffer for 1 hour at room temperature, then the product was incubated with Fluorescence for 0.5 hour at temperature. The activity of MAOA was detected by Promega MAO-GloTMAssay. |
