货号 | 31362 |
别名 | UBE2E1 |
使用方法 | Recombinant UbcH6 is suitable for use in ubiquitin conjugating assays. A good starting point is 25 to 100 ng of enzyme per assay. |
供应商 | Active Motif |
背景 | UBCH6 (Ubiquitin-Conjugating Enzyme E2E 1) Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes the covalent attachment of ISG15 to other proteins. Mediates the selective degradation of short-lived and abnormal proteins. In vitro also catalyzes Lys-48-linked polyubiquitination. |
运输条件 | Dry Ice |
存放说明 | Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
外观 | 50 µg recombinant UbcH6 expressed in E. coli supplied in a buffer of 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 200 mM imidazole, 20% glycerol, and 3 mM DTT. |
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UBCH6 protein gel. UBCH6 run on an SDS-PAGE gel and stained with Coomassie blue. | |
UBCH6 activity assay. Recombinant UbcH6 activity measured using a luminescent ubiquitin conjugating assay. |