| 货号 | 31358 |
| 别名 | HYPB; SETD2; HIF-1; HIP-1; KMT3A; HBP231; HSPC069; p231HBP |
| 使用方法 | Recombinant Set2 is suitable for use in histone methyltransferase assays. A good starting point is 50 to 500 ng of enzyme per assay. Specific Activity: 0.59 pmol/min/μg. This protein is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Assay conditions: Prepare a 50 μl reaction mix (50 mM TRIS pH 8.8, 5 mM MgCl2, 4 mM DTT, 20 μM S-adenosylmethionine, and varying amounts of SETD2) and add to substrate-coated wells. Incubate at room temperature for 1 hr. Add anti-methyl histone H3K36 antibody and incubate 1hr. Next, incubate 1h with the HRP-labeled secondary antibody followed by chemiluminescent detection. |
| 供应商 | Active Motif |
| 背景 | Set2 (SET Domain Containing 2) is a Histone methyltransferase that specifically trimethylates Lys-36 of histone H3 (H3K36me3) using dimethylated Lys-36 (H3K36me2) as substrate. Represents the main enzyme generating H3K36me3, a specific tag for epigenetic transcriptional activation. Plays a role in chromatin structure modulation during elongation by coordinating recruitment of the FACT complex and by interacting with hyperphosphorylated POLR2A. Acts as a key regulator of DNA mismatch repair in G1 and early S phase by generating H3K36me3, a mark required to recruit MSH6 subunit of the MutS alpha complex: early recruitment of the MutS alpha complex to chromatin to be replicated allows a quick identification of mismatch DNA to initiate the mismatch repair reaction. H3K36me3 also plays an essential role in the maintenance of a heterochromatic state, by recruiting DNA methyltransferase DNMT3A. H3K36me3 is also enhanced in intron-containing genes, suggesting that SETD2 recruitment is enhanced by splicing and that splicing is coupled to recruitment of elongating RNA polymerase. Required during angiogenesis. Recruited to the promoters of adenovirus 12 E1A gene in case of infection, possibly leading to regulate its expression. |
| 运输条件 | Dry Ice |
| 存放说明 | Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 外观 | 50 µg recombinant Set2 expressed in E. coli supplied at a concentration of 0.37 µg/µl in a buffer of 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 16 mM glutathione, 20% glycerol and 3 mM DTT. |
| 本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。 |
Recombinant Set2 protein gel. Set2 protein was run on an SDS-PAGE gel and stained with Coomassie blue. | |
Set2 activity assay. Recombinant Set2 activity measured using a chemiluminescent methyltransferase assay. |
