| 货号 | 31340 |
| 别名 | SIR2; hSIR2; SIR2L1 |
| 使用方法 | Recombinant SIRT1 is suitable for use in histone deacetylase assays. A recommended starting point for the HDAC assay is 25-100 ng of recombinant protein per reaction. |
| 供应商 | Active Motif |
| 背景 | SIRT1(Sirtuin 1,SIR2L1) is a member of the Sirtuin family of proteins related to the yeast Sir2 protein. Like Sir2 and other Sirtuins, SIRT1 is an NAD-dependent histone and protein deacetylase. SIRT1 catalyzes the removal of acetyl groups from a variety of protein substrates (NBS1, p53, Ku70, etc.) as well as from Histone H4 Lys16 (H4K16). SIRT1 is involved in the regulation of a number of cellular processes, including transcription, metabolism, DNA repair and aging, and increased SIRT1 is associated with extended lifespan of C. elegans. |
| 运输条件 | Dry Ice |
| 存放说明 | Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 外观 | Recombinant SIRT1 protein supplied at a concentration of 0.2 µg/µl in 20 mM Tris-HCl (pH7.3), 300 mM KCl, 0.2 mM EDTA and 20% glycerol. |
| 参考文献 |
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Recombinant SIRT1 protein gel. SIRT1 protein was run on an SDS-PAGE gel and stained with Coomassie blue. | |
Recombinant SIRT1 activity assay 25 ng of acetylated p53 (α-Acp53) was incubated with SIRT1 protein in reaction buffer including 40mM Tris pH 8.0, 5 mM MgCl2, 2mM NAD, and 140 mM KCl for 2 hour at 30c. |
