Recombinant TFIIA α/β protein
货号：
31330 基本售价：
5727.5 元 规格：
10 ug
产品信息
概述| 货号 | 31330 |
| 别名 | GTF2A1, TF2A1; TFIIA; TFIIAL; TFIIA-42 |
| 使用方法 | Recombinant TFIIA protein is suitable for use in in vitro transcription assays. We recommend to start with a range of protein concentrations (e.g. 5 ng, 50 ng, and 500 ng) to determine the optimal amount needed for each in vitro transcription assay. |
性能| 供应商 | Active Motif |
| 背景 | TFIIA (also known as TF2A1,GTF2A1,general transcription factor IIA subunit 1). TFIIA is a component of RNA polymerase II transcription complex. TFIIA associates with TATA-binding subunit (TBP) to regulate transcriptional activity.TFIIA binds directly to TBP and stabilizes its binding to DNA, perhaps through direct contacts of its own with the DNA. TFIIA acts as an anti-repressor, stabilizing TFIID binding by blocking repressors of transcription that inhibit binding of other transcription factors or that remove TBP from the DNA. Activation of transcription may be dependent on this TFIIA function. TFIIA is comprised of multiple subunits, TFIIA1 and TFIIA2. TFIIA1 has two subunits, TFIIA alpha and TFIIA beta, which are processed from the same polypeptide precursor. |
| 运输条件 | Dry Ice |
| 存放说明 | Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 外观 | 10 µg recombinant TFIIA protein is provided in 20 mM Tris-HCl (pH7.3), 300 mM KCl, 0.2 mM EDTA and 20% glycerol. Please refer to product insert upon arrival for lot-specific concentration. |
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