Recombinant KAT2A (GCN5) (362-837) protein
货号：
31204 基本售价：
5727.5 元 规格：
5 ug
产品信息
概述| 货号 | 31204 |
| 别名 | KAT2A; hGCN5; GCN5L2; PCAF-b |
| 使用方法 | This protein has been tested for activity in the fluorescent HAT Assay Kit, Catalog No. 56100. 50 ng of GCN5 were assayed per well using 50 µM histone H3 peptide substrate, 50 µM Acetyl-CoA and incubated for 30 minutes at room temperature. The assay was read on a fluorescent plate reader (ex. 360-390 nm, em. 450-470 nm). The specific activity was 100 pmol/min/µg. |
性能| 供应商 | Active Motif |
| 背景 | GCN5 (General control of nutrition 5), also known as K (lysine) acetyltransferase 2A (KAT2A), is a component of the ATAC complex, which is a complex that specifically exerts histone acetyltransferase (HAT) activity on histones H3 and H4. GCN5 acts as a histone acetyltransferase to promote transcriptional activation. In the case of HIV-1 infection, it is recruited by the viral protein TAT and regulates TATs transactivating activity. In addition, evidence suggests GCN5 may play a role in the induction of chromatin remodeling of proviral genes. GCN5 also functions as a repressor of NFκB by promoting ubiquitination of the NFκB subunit RelA in a HAT-independent manner. |
| 运输条件 | Dry Ice |
| 存放说明 | Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 外观 | 5 µg of Recombinant GCN5 protein, active. GCN5 is formulated in 20 mM Tris-HCl pH 7.3, 20% glycerol, 0.2 mM EDTA, 300 mM KCl, 1 mM DTT containing 0.5 mM PMSF. |
声明| 本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。 |
参考图片Recombinant GCN5 protein activity assay.
GCN5 activity assayed with the fluorescent HAT assay (Catalog No. 56100) using 50 ng of GCN5, 50 µM histone H3 peptide substrate (residues 5-23 of human H3), 50 µM Acetyl-CoA and 15 µM Anacardic Acid for inhibition. GCN5 was assayed alone to determine background and the resulting value was subtracted from this data. |
