货号 | 31202 |
别名 | YES1; c-yes; HsT441; P61-YES |
使用方法 | Recombinant Yes is suitable for kinase assays and Western blot. The molecular weight of the protein is ~90.997 kDa. The activity of the protein is ~ 29 pmol/µg min. Recommended kinase reaction conditions: 60 mM HEPES-NaOH, pH 7.5, 3 mM MgCl2, 3 mM MnCl2, 3 µM Na-orthovanadate, 1.2 mM DTT, ATP (variable), 2.5 µg/50 µl PEG20.000, Substrate: PolyEY, 1 µg / 50 µl, Recombinant Yes: 50 ng/50 µl. Kinase activity may vary depending on the substrate and reaction conditions used. |
供应商 | Active Motif |
运输条件 | Dry Ice |
外观 | 10 µg of Recombinant Yes protein in 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol. Protein is supplied at 0.116 µg/µl. |
本官网所有报价均为常温或者蓝冰运输价格,如有产品需要干冰运输,需另外加收干冰运输费。 |
Recombinant Yes protein tested by SDS-PAGE gel. 0.5 µg of Yes protein was analyzed using SDS/PAGE followed by Western blot with either an anti-Yes or anti-GST antibody. |