| 货号 | 31200 |
| 使用方法 | Recombinant VRK1 is suitable for kinase assays and Western blot. The molecular weight of the protein is ~75.142 kDa. The activity of the protein is 30 pmol/µgxmin. Recommended kinase reaction conditions: 60 mM HEPES-NaOH, pH 7.5, 3 mM MgCl2, 3 mM MnCl2, 3 μM Na-orthovanadate, 1.2 mM DTT, 50 μg / ml PEG20.000, ATP (variable), Substrate: R11-S6-Peptide, (R11-IAKRRRLSSLRASTSKSESSQK), 200 μg / μl, VRK1: 4.0 μg / ml Kinase activity may vary depending on the substrate and reaction conditions used. |
| 供应商 | Active Motif |
| 背景 | VRK1 (Vaccinia Related Kinase 1) is a serine/threonine kinase involved in Golgi disassembly during the cell cycle: following phosphorylation by PLK3 during mitosis, required to induce Golgi fragmentation. Acts by mediating phosphorylation of downstream target protein. Phosphorylates Thr-18 of p53/TP53 and may thereby prevent the interaction between p53/TP53 and MDM2. Phosphorylates casein and histone H3. Phosphorylates BANF1: disrupts its ability to bind DNA, reduces its binding to LEM domain-containing proteins and causes its relocalization from the nucleus to the cytoplasm. Phosphorylates ATF2 which activates its transcriptional activity. |
| 运输条件 | Dry Ice |
| 存放说明 | Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 外观 | 10 µg of Recombinant VRK1 protein in 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol. Protein is supplied at 0.269 µg/µl. |
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Recombinant VRK1 protein tested by SDS-PAGE gel. 2 µg of VRK1 protein was analyzed using SDS/PAGE followed by Western blot with an anti-GST antibody. |
