| 货号 | 31195 |
| 别名 | ASV, SRC1, c-SRC, p60-Src |
| 使用方法 | Recombinant Src is suitable for kinase assays and Western blot. The molecular weight of the protein is ~89.744 kDa. The activity of the protein is ~ 142 pmol/µg min. Recommended kinase reaction conditions: 60 mM HEPES-NaOH, pH 7.5, 3 mM MgCl2, 3 mM MnCl2, 3 µM Na-orthovanadate, 1.2 mM DTT, ATP (variable), 2.5 µg/50 µl PEG20.000, Substrate: PolyEY, 1 µg / 50 µl, Recombinant Src: 20 ng/50 µl. Kinase activity may vary depending on the substrate and reaction conditions used. |
| 供应商 | Active Motif |
| 背景 | The proto-oncogene tyrosine-protein kinase Src is a non-receptor protein tyrosine protein kinase that is involved in different signal transduction pathways that regulate such vital cellular processes as cell-cell interactions, cell migration, transcription and proliferation through the phosphorylation of many substrates. It is one of eight members of the Src family of non-receptor tyrosine kinases (Src, Fyn, Yes, Lck, Lyn, Hck, Fgr and Blk) that interact with the intracellular domains of growth factor/cytokine receptors, GPCRs and integrins. Members of the Src kinase family have a very similar domain structure with a high degree of homology in the SH1(catalytic), linker, SH2 (p-Tyr binding), SH3 (protein-protein interaction) and SH4 (membrane association) domains. c-Src, Fyn and Yes are ubiquitously expressed, although high levels of c-Src are found in platelets, neural tissue and osteoclasts. The activity of the Src kinase family can also be regulated by phosphatases (e.g. SHP1), binding to adaptor proteins (e.g. CBP) and proteasomal degradation. Src kinases are key upstream mediators of both the PI 3-K and MAPK signaling pathways, and have been shown to have importantroles in cell proliferation, migration and survival. |
| 运输条件 | Dry Ice |
| 外观 | 10 µg of Recombinant Src protein in 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol. Protein is supplied at 0.147 µg/µl. |
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Recombinant Src protein tested by SDS-PAGE gel. 2 µg of Src protein was analyzed using SDS/PAGE followed by Western blot with either an anti-Src or anti-GST antibody. |
