| 货号 | 31192 |
| 使用方法 | Recombinant PDK1 is suitable for kinase assays and Western blot. The molecular weight of the protein is ~81.331 kDa. The activity of the protein is ~ 6 pmol/µg min. Recommended kinase reaction conditions: 60 mM HEPES-NaOH, pH 7.5, 3 mM MgCl2, 3 mM MnCl2, 3 µM Na-orthovanadate, 1.2 mM DTT, ATP (variable), 2.5 µg/50 µl PEG20.000, Substrate: tetra(LRRWSLG), 2.5 µg / 50 µl, Recombinant PDK1: 50 ng/50 µl. Kinase activity may vary depending on the substrate and reaction conditions used. |
| 供应商 | Active Motif |
| 运输条件 | Dry Ice |
| 外观 | 10 µg of Recombinant PDK1 protein in 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol. Protein is supplied at 0.27 µg/µl. |
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Recombinant PDK1 protein tested by SDS-PAGE gel. 0.5 µg of PDK1 protein was analyzed using SDS/PAGE followed by Western blot with either an anti-PDK1 or anti-GST antibody. |
