| 货号 | 31188 |
| 别名 | P21 (RAC1) Activated Kinase 2, PAK65, PAKgamma, PAKgamma |
| 使用方法 | Recombinant PAK2 is suitable for kinase assays and Western blot. The molecular weight of the protein is ~87.954 kDa. The activity of the protein is ~ 228 pmol/µg min. Recommended kinase reaction conditions: 60 mM HEPES-NaOH, pH 7.5, 3 mM MgCl2, 3 mM MnCl2, 3 µM Na-orthovanadate, 1.2 mM DTT, ATP (variable), 2.5 µg/50 µl PEG20.000, Substrate: tetra (LRRWSLG), 5 µg / 50 µl, Recombinant PAK2 : 50 ng/50 µl. Kinase activity may vary depending on the substrate and reaction conditions used. |
| 供应商 | Active Motif |
| 背景 | The p21 activated kinases (PAK) are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. The PAK proteins are a family of serine/threonine kinases that serve as targets for the small GTP binding proteins, CDC42 and RAC1, and have been implicated in a wide range of biological activities. The protein encoded by this gene is activated by proteolytic cleavage during caspase-mediated apoptosis, and may play a role in regulating the apoptotic events in the dying cell. |
| 运输条件 | Dry Ice |
| 外观 | 10 µg of Recombinant PAK2 protein in 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol. Protein is supplied at 0.403 µg/µl. |
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Recombinant PAK2 protein tested by SDS-PAGE gel. 0.5 µg of PAK2 protein was analyzed using SDS/PAGE followed by Western blot with either an anti-PAK2 or anti-GST antibody. |
