| 货号 | 31186 |
| 别名 | Mitogen-Activated Protein Kinase-Activated Protein Kinase 5, MK5, MK-5, PRAK, MAPKAP-K5 |
| 使用方法 | Recombinant MAPKAPK5 is suitable for kinase assays and Western blot. The molecular weight of the protein is ~84.116 kDa. The activity of the protein is ~ 2 pmol/µg min. Recommended kinase reaction conditions: 60 mM HEPES-NaOH, pH 7.5, 3 mM MgCl2, 3 mM MnCl2, 3 µM Na-orthovanadate, 1.2 mM DTT, ATP (variable), 2.5 µg/50 µl PEG20.000, Substrate: tetra (LRRWSLG), 3 µg / 50 µl, Recombinant MAPKAPK5 : 200 ng/50 µl. Kinase activity may vary depending on the substrate and reaction conditions used. |
| 供应商 | Active Motif |
| 运输条件 | Dry Ice |
| 外观 | 10 µg of Recombinant MAPKAPK5 protein in 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol. Protein is supplied at 0.113 µg/µl. |
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Recombinant MAPKAPK5 protein tested by SDS-PAGE gel. 0.5 µg of MAPKAPK5 protein was analyzed using SDS/PAGE followed by Western blot with either an anti-MAPKAPK5 or anti-GST antibody. |
