货号 | 31183 |
别名 | Mitogen-Activated Protein Kinase 10, MAPK10, JNK3A, PRKM10, SAPK1b, p493F12, p54bSAPK |
使用方法 | Recombinant JNK3 is suitable for kinase assays and Western blot. The molecular weight of the protein is ~82.161 kDa. The activity of the protein is ~ 0.2 pmol/µg min. Recommended kinase reaction conditions: 60 mM HEPES-NaOH, pH 7.5, 3 mM MgCl2, 3 mM MnCl2, 3 µM Na-orthovanadate, 1.2 mM DTT, ATP (variable), 2.5 µg/50 µl PEG20.000, Substrate: Rb-CTF 2 µg / 50 µl, Recombinant JNK3 : 200 ng/50 µl. Kinase activity may vary depending on the substrate and reaction conditions used. |
供应商 | Active Motif |
运输条件 | Dry Ice |
外观 | 10 µg of Recombinant JNK3 protein in 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol. Protein is supplied at 0.126 µg/µl. |
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Recombinant JNK3 protein tested by SDS-PAGE gel. 0.5 µg of JNK3 protein was analyzed using SDS/PAGE followed by Western blot with either an anti-JNK3 or anti-GST antibody. |