| 货号 | 31174 |
| 别名 | JTK9, p59Hck, p61Hck, HCK Proto-Oncogene, Src Family Tyrosine Kinase |
| 使用方法 | Recombinant HCK is suitable for kinase assays and Western blot. The molecular weight of the protein is ~86.707 kDa. The activity of the protein is ~ 34 pmol/µg min. Recommended kinase reaction conditions: 60 mM HEPES-NaOH, pH 7.5, 3 mM MgCl2, 3 mM MnCl2, 3 µM Na-orthovanadate, 1.2 mM DTT, ATP (variable), 2.5 µg/50 µl PEG20.000, Substrate: PolyEY 1 µg/50 µl, Recombinant HCK: 200 ng/50 µl. Kinase activity may vary depending on the substrate and reaction conditions used. |
| 供应商 | Active Motif |
| 运输条件 | Dry Ice |
| 外观 | 10 µg of Recombinant HCK protein in 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol. Protein is supplied at 0.143 µg/µl. |
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Recombinant HCK protein tested by SDS-PAGE gel. 0.5 µg of HCK protein was analyzed using SDS/PAGE followed by Western blot with an anti-GST antibody. |
