货号 | 31160 |
别名 | ABL, c-Abl, JTK7, p150, v-Abl, bcr/abl, c-ABL1 |
使用方法 | Recombinant ABL1 is suitable for kinase assays and Western blot. The molecular weight of the protein is ~74.14 kDa. The activity of the protein is ~ 33 pmol/µg min. Recommended kinase reaction conditions: 60 mM HEPES-NaOH, pH 7.5, 3 mM MgCl2, 3 mM MnCl2, 3 µM Na-orthovanadate, 1.2 mM DTT, ATP (variable), 2.5 µg/50 µl PEG20.000, Substrate: PolyAEKY, 5 µg/50 µl, Recombinant ABL1: 50 ng/50 µl. Kinase activity may vary depending on the substrate and reaction conditions used. |
供应商 | Active Motif |
运输条件 | Dry Ice |
外观 | 10 µg of Recombinant ABL1 protein in 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol. Protein is supplied at 0.206 µg/µl. |
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Recombinant ABL1 protein tested by SDS-PAGE gel. 0.5 µg of ABL1 protein was analyzed using SDS/PAGE followed by Western blot with either an anti-ABL1 or anti-GST antibody. |