Recombinant Mononucleosomes (TH2B) - biotinylated
货号：
31557 基本售价：
6090.0 元 规格：
20 ug
产品信息
概述| 货号 | 31557 |
| 别名 | TH2B, testis-specific H2B, testis-specific Histone 2B |
| 使用方法 | Recombinant Mononucleosomes (TH2B) - biotinylated are suitable for use as substrates in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. |
性能| 供应商 | Active Motif |
| 背景 | In vivo, the nucleosome is the basic structural unit of chromatin. It is comprised of about 146 bp of DNA wrapped around a core of eight histones of four different types: H2A, H2B, H3 and H4. Histones are subject to posttranslational modifications, such as methylation, acetylation, phosphorylation and mono-ubiquitination.Histone modifications influence multiple chromatin-templated processes such as gene transcription, DNA repair and recombination. Besides the “major“ histones, there are some histone variants in specific regions of chromatin or in specific cell types. Histone variants are involved in multiple biology processes including chromosome segregation, DNA repair, transcriptional regulation and mRNA processing.
TH2B (testis-specific H2B) is originally identified as a testis-specific variant of histone H2B. TH2A is a testis-specific variant of histone H2A. They are abundant in the testis, oocytes and fertilized eggs. TH2A and TH2B are controlled by a shared promoter that is located between them. Disruption of Th2aandTh2b genes causes defects in spermatogenesis. TH2A/TH2B may enhance reprogramming by introducing processes that normally operate in zygotes and during SCNT. TH2A and TH2B induce nucleosome instability. TH2B controls the chromatin-to-nucleoprotamine transition.
Nucleosomes are more physiologically relevant substrates than histones and histone-derived peptides for in vitro studies. More importantly, some histone methyltransferases are significantly more active, as well as specific, when using nucleosomal substrates in HMT assays, such as DOT1L and NSD family enzymes. Nucleosomes are also widely used in histone methyltransferase screening assays to identify small molecular inhibitors for drug discovery. |
| 运输条件 | Dry Ice |
| 存放说明 | Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 外观 | Recombinant Mononucleosomes (TH2B) - biotinylated (20 µg protein + 20 µg DNA) are supplied in 10 mM Tris-HCl, pH 8.0, 1 mM EDTA, 2 mM DTT and 20% glycerol. Please refer to product insert upon arrival for lot-specific concentration. |
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