Recombinant SIRT3 (102-399) protein
货号：
31529 基本售价：
5872.5 元 规格：
100 ug
产品信息
概述| 货号 | 31529 |
| 别名 | SIRT-3, Sirtuin 3, Sir1-Like 3, EC 3.5.1 |
| 使用方法 | Recombinant SIRT3 protein is useful for histone and other protein deacetylase assays and the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
HDAC Activity Assay Conditions: 3 µM histone peptide H3K9ac (1-21aa) was incubated with SIRT3 (102-399) in reaction buffer including 25 mM Tris-HCl pH 8.0, 137 mM NaCl, 2.7 mM MgCl2, 1 mM KCl, 0.1 mg/ml BSA and 50 µM NAD+ for 30 min at 37°C. HTRF assay was used for activity detection. |
性能| 供应商 | Active Motif |
| 背景 | SIRT3 (Sirtuin 3) is a member of the SIR2 / Sirtuin family of NAD+-dependant deacetylase enzymes. Sirtuin family enzymes can be found in the cytoplasm, mitochondria or nucleus of a cell and are ubiquitously expressed. SIRT3 has multiple targets for deacetylation, including ACSS1, IDH, GDH, SOD2, PDHA1, LCAD, SDHA and the ATP synthase subunit ATP5O. SIRT3 contributes to the regulation of the cellular energy metabolism and tissue-specifc ATP levels. |
| 运输条件 | Dry Ice |
| 存放说明 | Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 外观 | Recombinant SIRT3 (102-399) protein is supplied in 25 mM Tris pH 8.0, 150 mM NaCl, 5% glycerol. Please refer to product insert upon arrival for lot-specific concentration. Please refer to product insert upon arrival for lot-specific concentration. |
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