| 货号 | 31574 |
| 别名 | nuclear receptor binding SET domain protein 1, WHSC1, MMSET, TRX5 |
| 使用方法 | Recombinant NSD2-SET (E1099K) is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. Protein Function: H3K36me2 methyltransferase activity. HMT Assay Conditions: 60 ng/µl recombinant nucleosomes was incubated with 30 ng/µl NSD2-SET (E1099K) protein in reaction buffer including 50 mM TrisCl, pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP, 50 µM SAM, for 3 hours at room temperature. Activity was detected by Western Blot. |
| 供应商 | Active Motif |
| 背景 | NSD2 (nuclear receptor binding SET domain protein 1; also known as WHSC1; MMSET; TRX5) is a SET domain-containing histone methyltransferase specific for lysine 36 of histone H3. The NSD2 gene is deleted in Wolf-Hirschhorn syndrome, a disorder characterized by developmental defects and mental retardation. In 15% of patients with Multiple Myeloma (MM), a chromosomal translocation leads to NSD2 gene being aberrantly placed under the control of the IgH intronic µ enhancer and expressed at very high levels. This event is apparently a key component in cellular transformation leading to MM. Reduction in NSD2 expression in a myeloma cell line suppressed its growth, suggesting a role for NSD2 in maintaining the transformed state. NSD2 p.Glu1099Lys (p.E1099K) variant was identified in nontranslocated acute lymphoblastic leukemia (ALL) cell lines. |
| 运输条件 | Dry Ice |
| 存放说明 | Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 外观 | Recombinant NSD2-SET (E1099K) protein is suppled in 50 mM Tris pH 8.0, 300 mM NaCl, 5% glycerol. Please refer to product insert upon arrival for lot-specific concentration. |
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