Recombinant JARID1B / KDM5B (2-751) protein
货号：
31518 基本售价：
5872.5 元 规格：
20 ug
产品信息
概述| 货号 | 31518 |
| 别名 | Lysine Demethylase 5B, Cancer/Testis Antigen 31, Retinoblastoma-Binding Protein 2 Homolog 1, Histone Demethylase JARID1B |
| 使用方法 | Recombinant JARID1B / KDM5B (2-751) is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiing.
HDM Activity Assay Conditions: 3 µM H3K4me3 (1-21aa) peptide was incubated with KDM5B (2-751) protein in reaction buffer including 50 mM HEPES pH 7.5, 0.02% Triton X-100, 100 µM 2-OG, 100 µM ascorbate, 50 µM (NH4)2Fe(SO4)2·6H2O for 1 hour at room temperature. HTRF assay and MALDI-TOF was used for activity detection. |
性能| 供应商 | Active Motif |
| 背景 | Lysine (K)-specific demethylase 5B (KDM5B), also known as Jumonji, AT rich interactive domain 1B (JARID1B), is a histone demethylase that specifically demethylates lysine 4 of histone H3 (H3K4), thereby playing a central role in defining the histone code. KDM5B demethylates trimethylated, dimethylated and monomethylated H3K4. It does not demethylate histone H3K9 or H3K27. KDM5B functions as a transcriptional corepressor for FOXG1B and PAX9. KDM5B induces the proliferation of breast cancer cells by repressing tumor suppressor genes such as BRCA1 and HOXA5. In contrast, it may act as a tumor suppressor for melanoma. |
| 运输条件 | Dry Ice |
| 存放说明 | Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 外观 | Recombinant JARID1B / KDM5B (2-751) protein was expressed in Sf9 cells and is supplied in 25 mM HEPES pH 7.5, 300 mM NaCl, 5% glycerol, 0.04% Triton X-100, 0.2 mM TCEP. Please refer to product insert upon arrival for lot-specific concentration. |
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