| 货号 | 10006809-50mg |
| 描述 | Thioester analogs of glycerophospholipids, in combination with Ellman’s reagent, are convenient colorimetric substrates for the measurement of phospholipase (PL) activity.1,2 Diheptanoyl thio-PC is a commonly-used colorimetric substrate for all PLA2s, with the exception of cPLA2 and platelet-activating factor acetylhydrolase (PAF-AH).3 Heptanoyl thio-PC is an analog of diheptanoyl thio-PC that contains an ether-linked saturated C16 moiety at the sn-1 position rather than a heptanoyl thiol ester. Porcine pancreatic and bee venom sPLA2 enzymes exhibit 10-13 fold less activity when assayed with heptanoyl thio-PC compared to diheptanoyl thio-PC.4 This decrease in activity has not been thoroughly investigated. |
| 供应商 | Cayman |
| 应用文献 | |
| 1.Hendrickson, H.S.,Hendrickson, E.K., and Dybvig, R.H. Chiral synthesis of a dithiolester analog of phosphatidylcholine as a substrate for the assay of phospholipase A2. Journal of Lipid Research 24, 1532-1537 (1983). 2.Reynolds, L.J.,Hughes, L.L., and Dennis, E.A. Analysis of human synovial fluid phospholipase A2 on short chain phosphatidylcholine-mixed micelles: Development of a spectrophotometric assay suitable for a microtiterplate reader. Analytical Biochemistry 204, 190-197 (1992). 3.Roberts, M.F. Phospholipases: Structural and functional motifs for working at an interface. The FASEB Journal 10, 1159-1172 (1996). 4.Johnson, J. Personal Communication. (2005). | |
| 运输条件 | Room temperature in continental US; may vary elsewhere |
| 存放说明 | -20 |
| 纯度 | ≥98% |
| 计算分子量 | 609.9 |
| 分子式 | C31H64NO6PS |
| 稳定性 | ≥ 1 year |
| 本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。 |