StemMACS Cas9 Nickase mRNA
货号：
130-107-679 基本售价：
询价 规格：
EA
产品信息
概述| 货号 | 130-107-679 |
| 描述 | StemMACS™ Cas9 Nickase mRNA encodes a D10A mutated version of Cas9 from Streptomyces pyogenes for transfection. |
性能| 供应商 | Miltenyi |
| 背景 | Cas9 originates from the CRISPR (Clustered Regularly Interspersed Palindromic Repeats) adaptive immunity system in Streptococcus pyogenes. The original Cas9 Nuclease has two catalytic domains, the RuvC-like nuclease domain that cleaves the non-complementary strand and the HNH nuclease domain that cleaves the complementary strand of DNA. Cas9 Nickase carries a D10A point mutation that eliminates the nuclease function of the RuvC-like domain, so that only the strand of the DNA that is complementary to the guide RNA sequence is cleaved. |
