ProLong®Gold Antifade Reagent with DAPI
货号：
8961S 基本售价：
2372.0 元 规格：
10ml
产品信息
概述| 货号 | 8961S |
| 应用 | IF-P/IF-F/IF-IC |
性能| 供应商 | CST |
| 背景 | ProLong® Gold Antifade Reagent with DAPI offers enhanced resistance to photobleaching, is premixed and ready to use, and causes little or no quenching of the fluorescent signal. The reagent cures within 24 hours and samples can be saved for months after mounting. This reagent is compatible with most dyes and is appropriate for use in multi-color labeling assays.The inclusion of DAPI within this mounting reagent eliminates the need for a separate nuclear counterstaining step. 4, 6-diamidino-2-phenylindole, dihydrochloride (DAPI) is a blue fluorescent DNA dye that targets double-stranded AT clusters in the DNA minor groove (1). One molecule of dye binds to each 3 base pairs of dsDNA and yields an approximate 20-fold fluorescent enhancement (2). The level of DAPI-DNA fluorescence is proportional to DNA content (3). |
| 存放说明 | -20C |
参考图片Epifluorescent analysis of HeLa cells using COX IV (3E11) Rabbit mAb (Alexa Fluor® 488 Conjugate) #4853 (green) and ProLong® Gold Antifade Reagent with DAPI (blue pseudocolor).外荧光分析HeLa细胞，使用COX IV (3E11) Rabbit mAb (Alexa Fluor® 488 Conjugate) #4853（绿色）和ProLong® Gold Antifade Reagent with DAPI（蓝色假色）。 |
Confocal immunofluorescent analysis of COS-7 cells, mounted in either glycerol (upper panels) or ProLong® Gold Antifade Reagent #9071 (lower panels), using β-Actin (8H10D10) Mouse mAb #3700 and Anti-mouse IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 555 Conjugate) #4409 (red) (0, 3.3, or 10 min of imaging).共聚焦免疫荧光分析COS-7细胞，用甘油（上面）或ProLong® Gold Antifade Reagent #9071（下面）封固，使用的抗体是：β-Actin (8H10D10) Mouse mAb #3700和Anti-mouse IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 555 Conjugate) #4409（红色）(成像0, 3.3,或10分钟)。 |
