| 货号 | 8118S |
| 供应商 | CST |
| 背景 | Met, a high affinity tyrosine kinase receptor for hepatocyte growth factor (HGF, also known as scatter factor) is a disulfide-linked heterodimer made of 45 kDa α- and 145 kDa β-subunits (1,2). The α-subunit and the amino-terminal region of the β-subunit form the extracellular domain. The remainder of the β-chain spans the plasma membrane and contains a cytoplasmic region with tyrosine kinase activity. Interaction of Met with HGF results in autophosphorylation at multiple tyrosines, which recruit several downstream signaling components, including Gab1, c-Cbl, and PI3 kinase (3). These fundamental events are important for all of the biological functions involving Met kinase activity. The addition of a phosphate at cytoplasmic Tyr1003 is essential for Met protein ubiquitination and degradation (4). Phosphorylation at Tyr1234/1235 in the Met kinase domain is critical for kinase activation. Phosphorylation at Tyr1349 in the Met cytoplasmic domain provides a direct binding site for Gab1 (5). Altered Met levels and/or tyrosine kinase activities are found in several types of tumors, including renal, colon, and breast. Thus, Met is an attractive cancer therapeutic and diagnostic target (6,7). |
| 存放说明 | 4C |
| 参考文献 | Cooper, C.S. et al. (1984) Nature 311, 29-33. Bottaro, D.P. et al. (1991) Science 251, 802-4. Bardelli, A. et al. (1997) Oncogene 15, 3103-11. Taher, T.E. et al. (2002) J Immunol 169, 3793-800. Schaeper, U. et al. (2000) J Cell Biol 149, 1419-32. Eder, J.P. et al. (2009) Clin Cancer Res 15, 2207-14. Sattler, M. and Salgia, R. (2009) Update Cancer Ther 3, 109-118. |
左，未经处理；右，经SU11274刺激。 | |
Immunohistochemical analysis of paraffin-embedded MKN45 cell pellets, control (left) or SU11274-treated (right), using Phospho-Met (Tyr1234/1235) (D26) XP® Rabbit mAb #3077. |
