SignalSlide® Phospho-EGF Receptor IHC Controls【科瑞奥博】

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SignalSlide® Phospho-EGF Receptor IHC Controls

货号： 8102S 基本售价： 1776.0 元规格： 1Pack

产品信息

概述
货号	8102S

性能
供应商	CST
背景	The epidermal growth factor (EGF) receptor is a transmembrane tyrosine kinase that belongs to the HER/ErbB protein family. Ligand binding results in receptor dimerization, autophosphorylation, activation of downstream signaling, internalization, and lysosomal degradation (1,2). Phosphorylation of EGF receptor (EGFR) at Tyr845 in the kinase domain is implicated in stabilizing the activation loop, maintaining the active state enzyme, and providing a binding surface for substrate proteins (3,4). c-Src is involved in phosphorylation of EGFR at Tyr845 (5). The SH2 domain of PLCγ binds at phospho-Tyr992, resulting in activation of PLCγ-mediated downstream signaling (6). Phosphorylation of EGFR at Tyr1045 creates a major docking site for c-Cbl, an adaptor protein that leads to receptor ubiquitination and degradation following EGFR activation (7,8). The GRB2 adaptor protein binds activated EGFR at phospho-Tyr1068 (9). A pair of phosphorylated EGFR residues (Tyr1148 and Tyr1173) provides a docking site for the Shc scaffold protein, with both sites involved in MAP kinase signaling activation (2). Phosphorylation of EGFR at specific serine and threonine residues attenuates EGFR kinase activity. EGFR carboxy-terminal residues Ser1046 and Ser1047 are phosphorylated by CaM kinase II; mutation of either of these serines results in upregulated EGFR tyrosine autophosphorylation (10).
存放说明	4C
参考文献	Hackel, P.O. et al. (1999) Curr Opin Cell Biol 11, 184-9. Zwick, E. et al. (1999) Trends Pharmacol Sci 20, 408-12. Cooper, J.A. and Howell, B. (1993) Cell 73, 1051-4. Hubbard, S.R. et al. (1994) Nature 372, 746-54. Biscardi, J.S. et al. (1999) J Biol Chem 274, 8335-43. Emlet, D.R. et al. (1997) J Biol Chem 272, 4079-86. Levkowitz, G. et al. (1999) Mol Cell 4, 1029-40. Ettenberg, S.A. et al. (1999) Oncogene 18, 1855-66. Rojas, M. et al. (1996) J Biol Chem 271, 27456-61. Feinmesser, R.L. et al. (1999) J Biol Chem 274, 16168-73.

参考图片

左，未经EGF刺激的KYSE450细胞；右，经过EGF刺激的KYSE450细胞。

Immunohistochemical analysis of paraffin-embedded KYSE450 cells, untreated (left) or treated with EGF (right), using Phospho-EGF Receptor (Tyr1068) (D7A5) XP (TM) Rabbit mAb #3777.用抗磷酸化EGFR（Tyr1068）的兔源单克隆抗体(D7A5) XP (TM) #3777对石蜡包埋的经过（右）和未经（左）EGF刺激的KYSE450细胞进行免疫组化检测。