| 货号 | 4087S |
| 应用 | IF-P/IF-F/F |
| 使用方法 | IF-F () IF-IC () IF-P () |
| 供应商 | CST |
| 背景 | Propidium Iodide (PI) is a fluorescent dye which intercalates between bases and stains both DNA and RNA. Specific DNA staining is achieved by enzymatic removal of RNA with a ribonuclease (RNase). PI/RNase is commonly used as a nuclear stain in fluorescent microscopy and as a DNA content determinant in cell cycle analyses by flow cytometry. Cells in G2 and M phases of the cell cycle contain twice the DNA content compared to those in G0 and G1 phases. DNA content during S phase lies between these extremes. PI/RNase Staining Solution has excitation and emission maxima of 535 and 617 nm, respectively (orange to red range of the spectrum). This staining solution can be used in tandem with antibodies without binding interference and is a suitable DNA stain for multiplex assays(1-3). |
| 存放说明 | 4C |
Confocal immunofluorescent analysis of HeLa cells using α-Tubulin (DM1A) Mouse mAb #3873 (green). Red = Propidium Iodide (PI)/RNase Staining Solution.激光共聚焦免疫荧光分析HeLa细胞，所用抗体为α-Tubulin (DM1A) Mouse mAb #3873 (绿色). 红色为碘化丙啶(PI )/核糖核酸酶染色试剂。 | |
Confocal immunofluorescent analysis of rat cerebellum using S6 Ribosomal Protein (54D2) Mouse mAb (Alexa Fluor® 647 Conjugate) #5548 (blue pseudocolor) and β3-Tubulin (D71G9) XP® Rabbit mAb #5568 (green). Red = Propidium Iodide (PI)/RNase Staining Solution.激光共聚焦免疫荧光分析大鼠小脑细胞，所用抗体为S6 Ribosomal Protein (54D2) Mouse mAb (Alexa Fluor ® 647 Conjugate) #5548 (blue pseudocolor) 和 β3-Tubulin (D71G9) XP ® Rabbit mAb #5568 (绿色)，红色为碘化丙啶(PI )/核糖核酸酶染色试剂（DNA） | |
Flow cytometric analysis of untreated Jurkat cells, using Phospho-Histone H3 (Ser10) Antibody (Alexa Fluor® 488 Conjugate) #9708 and Propidium Iodide (PI)/RNase Staining Solution (DNA content).流式细胞术分析未处理的Jurkat 细胞，所用抗体为Phospho-Histone H3 (Ser10) Antibody (Alexa Fluor® 488 Conjugate) #9708 和 碘化丙啶 (PI)/RNase Staining Solution (DNA content). |
