| 货号 | AP-9007-005 |
| 描述 |
Storage Conditions: Store at 4°C. Stable for 12 months when stored at 2° to 8°C. |
| 供应商 | Labvision |
| 存放说明 | 2-8摄氏度 |
| 操作步骤 | 1. Place five-micron thick tissue sections on glass slides coated with poly L-lysine or APTES. 2. Deparaffinize and rehydrate sections as usual. 3. Block endogenous peroxidase as usual. 4. Wash sections in wash-buffer for 2 x 5 minutes. 5. Cover sections with the pepsin solution (usually 0.2mL per section) and place the slides at 37°C for 10 minutes in a humidified chamber. 6. Wash sections in wash buffer for 2 x 5 minutes. 7. Block nonspecific sites with normal serum as usual. 8. Place optimally diluted primary antibody on the sections (incubation time and temperature for a given set of experimental conditions should be determined by the investigator). 9. Wash sections in buffer for 2 x 5 minutes. 10. Remaining procedure is same as routinely performed in your laboratory. |
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