MultiVision AP and HRP polymers are innovative, patented technologies consisting of smaller amino acid polymer subunits that minimize conflicts in binding the target protein. The smaller polymer allows for decreased binding conflicts, resulting in more consistent staining and better signal amplification.

• A primary antibody cocktail of choice may be used in conjunction with ready-to-use MultiVision polymer cocktail, composed of anti-mouse/ horseradish peroxidase (HRP) plus anti-rabbit/alkaline phosphatase (AP)
• Cocktail is specially formulated from MultiVision polymers that provide increased sensitivity, time-savings, and detection simplicity
• Yields higher sensitivity and antibody efficiency as well as better signal-to-noise ratios
• Biotin-free, eliminating background staining

• Antigen Specificity: anti-Mouse IgG (H+L) and anti-Rabbit IgG (H+L)

• Enzyme: Peroxidase and Phosphatase

• Chromogen/Substrate: LVBlue/ LVRed

Includes:

TL-012-MHRA: 1 MultiVision anti-rabbit/AP + anti-mouse/HRP polymer (cocktail) (TL-012-RAM), 1 Ultra V block (TA-012-UB), 1 Hydrogen Peroxidase Block (TA-012-HP), 1 LVBlue AP Buffer (TA-075-TMM), 1 LVBlue Chromogen Vial 1 (TA-001-AB1), 1 LVBlue Chromogen Vial 2 (TA-001-AB2), 1 LVBlue Chromogen Vial 3 (TA-001-AB3), 1 LVRed Chromogen Vial 1 (TA-004-HR1), 1 LVRed Chromogen Vial 2 (TA-002-HR2), 1 LVRed Chromogen Vial 3 (TA-002-HR3), 1 LVRed Chromogen Vial 4 (TA-002-HR4), 1 Mounting Media (TA-005-UA)

Note:

MultiVision Polymer Detection System may be employed with any primary antibody cocktail to visualize the presence of two proteins simultaneously. After the specific antibodies are bound to their respective antigens in tissue sections, the rabbit and mouse primary antibodies are located by a specially formulated cocktail of secondary antibody polymers: anti-mouse/ horseradish peroxidase (HRP) + anti-rabbit/alkaline phosphatase (AP). In general, the amino acid polymer is conjugated to AP or HRP enzymes and the Fab fragments of goat anti-rabbit or goat anti-mouse immunoglobulins. The polymer complex is then visualized with first the LVBlue chromogen for AP activity and after washing with the LVRed chromogen for HRP activity. A weak hematoxilin nuclear counterstain is not recommended.

1. Immunoenzyme Multiple Staining Methods. CM van der Loos, Handbook no. 45, BIOS Scientific Publishers, Oxford, UK, 1999. ISBN: 1-85996-187-8 or Springer-Verlag, New York, ISBN: 0-387-91594-x.

2. Multiple staining in molecular morphology, CM van der Loos. In: Molecular morphology in human tissues (Eds. Hacker GW and Tubbs RR), CRC Press, Boca Raton, FL, 2005, pp. 27-63.