| 货号 | 61537 |
| 别名 | PRO9856, LAVS3040 |
| 同种亚型 | IgG |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | ChIP/ChIP-Seq/Western Blot/Immunoprecipitation |
| 使用方法 | Validated Applications: ChIP: 5 - 10 µl per ChIP ChIP-Seq: 5 - 10 µl each IP: 10 µl per IP WB: 1:500 - 1:2,000 dilution ChIP-Seq validation was performed by Active Motifs Epigenetics Services and the complete data set is available in the UCSC Genome Browser by clicking here. |
| 供应商 | Active Motif |
| 纯化方式 | Affinity Purified |
| 免疫原 | This antibody was raised against a peptide within the C-terminal region of human BRD9. |
| 背景 | BRD9 (bromodomain containing 9) belongs to the BET subclass of proteins, which are characterized by two N-terminal bromodomains and one ET (Extra Terminal) domain. BRDs associate with chromatin through their bromodomains that recognize acetylated histone lysine residues. Bromodomains function as ‘readers’ of these epigenetic histone marks and regulate chromatin structure and gene expression by linking associated proteins to the acetylated nucleosomal targets. The ET domain functions as a protein binding motif and exerts atypical serine-kinase activity. The BET family consists of at least four members in mouse and human, BRD2 (also referred to as FSRG1, RING3), BRD3 (FSRG2, ORFX), BRD4 (FSRG4, MCAP/HUNK1), and BRDT (FSRG3, BRD6) that function in the regulation of transcriptional activation and chromatin remodeling. There are five isoforms of BRD9 that are produced by alternative splicing. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 80kDa |
| 参考文献 |
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BRD9 antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 30 µg of chromatin from a human synovial sarcoma cell line and 4 µl of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 11 million sequence tags were mapped to identify BRD9 binding sites. The image shows binding across a region of chromosome 2. You can view the complete data set in the UCSC Genome Browser, starting at this specific location, here. | |
BRD9 antibody (pAb) tested by Immunoprecipitation. 10 µl of BRD9 antibody was used to immunoprecipitate BRD9 from 250 µg of Raji nuclear cell extract (lane 2). 10 µl of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the BRD9 antibody at a dilution of 1:1,000. | |
BRD9 antibody (pAb) tested by Western blot. Nuclear extract (20 µg) of Raji cells (Lane 1) or nuclear extract (20 µg) of Caco-2 cells (Lane 2) probed with BRD9 antibody at a 1:1,000 dilution. |
