| 货号 | 61405 |
| 别名 | retinoblastoma binding protein 5, RBQ3, SWD1 |
| 同种亚型 | IgG |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | ChIP/ChIP-Seq/Western Blot |
| 使用方法 | Validated Applications: ChIP: 10 µl per ChIP ChIP-Seq: 10 µl each WB: 1:500 - 1:5,000 dilution ChIP-Seq validation was performed by Active Motifs Epigenetics Services and the complete data set is available in the UCSC Genome Browser by clicking here. |
| 供应商 | Active Motif |
| 纯化方式 | Affinity Purified |
| 免疫原 | This antibody was raised against a peptide within the C-terminal region of human RBBP5. |
| 背景 | As part of the MLL1/MLL complex, RBBP5 (retinoblastoma binding protein 5) is involved in methylation and dimethylation at Lys-4 of histone H3. H3 Lys-4 methylation represents a specific tag for epigenetic transcriptional activation. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. |
| 计算分子量 | 70 kDa |
| 参考文献 |
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RBBP5 antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 30 ug of human PBMC chromatin and 7 µl of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 21 million sequence tags were mapped to identify RBBP5 binding sites. The image shows binding across a region of chromosome 12. You can view the complete data set in the UCSC Genome Browser, starting at this specific location, here. | |
RBBP5 antibody (pAb) tested by Western blot. MCF-7 whole-cell extract (20 µg) probed with RBBP5 antibody at a dilution of 1:1,000. |
