| 货号 | 61269 |
| 别名 | SLEB10 |
| 同种亚型 | IgG |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | ChIP/ChIP-Seq/Western Blot |
| 使用方法 | Validated Applications: ChIP: 10 µl per ChIP ChIP-Seq & ChIP-chip: 10 µl each WB: 1:500 - 1:2,000 dilution The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required. |
| 供应商 | Active Motif |
| 纯化方式 | Affinity Purified |
| 免疫原 | This antibody was raised against a peptide within the N-terminal region of human IRF-5. |
| 背景 | IRF-5 (interferon regulatory factor 5) is a transcription factor involved in the induction of interferons IFNA and INFB and inflammatory cytokines upon virus infection. Activated by TLR7 or TLR8 signaling. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 60 kDa |
| 参考文献 |
| 本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。 |
IRF-5 antibody (pAb) tested by ChIP-Seq. (ChIP) was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) and 25 µg of chromatin from primary human macrophages + compound. ChIP DNA was sequenced on the Illumina HiSeq and 8 million sequence tags were mapped to identify IRF-5 binding sites across the genome. Both images show the expected enrichment. | |
IRF-5 antibody (pAb) tested by Western blot Nuclear extract of THP-1 cells (20 µg) probed with IRF-5 antibody (1:500). |
