| 货号 | 61211 |
| 别名 | BF1, BF2, QIN, FKH2, HBF2, HFK1, HFK2, HFK3, KHL2, FHKL3, FKHL1, FKHL2, FKHL3, FKHL4, HBF-1, HBF-2, HBF-3, FOXG1A, FOXG1B, FOXG1C, HBF-G2 |
| 同种亚型 | IgG |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | ChIP/ChIP-Seq/Western Blot |
| 使用方法 | Validated Applications: ChIP: 5 µl per ChIP ChIP-Seq: 5 µl each WB: 1:500 - 1:1,000 dilution ChIP-Seq validation was performed by Active Motifs Epigenetics Services and the complete data set is available in the UCSC Genome Browser by clicking here. For optimal results, primary antibody incubations should be performed at 4°C. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required. |
| 供应商 | Active Motif |
| 纯化方式 | Affinity Purified |
| 免疫原 | This FOXG1 antibody was raised against a peptide within the C-terminal region of human FOXG1. |
| 背景 | FOXG1 (Forkhead box protein G1, FKH2, FKHL1) is a transcriptional repressor that serves an important role in the development of the brain. FOXG1 is expressed in the neural plate early in development and is involved in the specification, proliferation and differentiation of the telencephalon. FOXG1 is also essential for proper patterning of the eye and development of the olfactory epithelium. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 55 kDa |
| 参考文献 |
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FOXG1 antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 30 ug of mouse whole brain chromatin and 2 µl of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 20 million sequence tags were mapped to identify FOXG1 binding sites. The image shows binding across a region of chromosome 4. You can view the complete data set in the UCSC Genome Browser, starting at this specific location, here. | |
FOXG1 antibody (pAb) tested by Western blot. Detection of Goosecoid by Western blot analysis. Lane 1: Whole-cell extract of Hek 293 cells (40 µg). Lane 2: Whole-cell extract of Jurkat cells (40 µg). Both were probed with FOXG1 antibody (pAb) at a 1:500 dilution. |
