| 货号 | 61177-AM |
| 别名 | NR1H2, NER, UNR, LXRB, NER-I, RIP15 |
| 同种亚型 | IgG |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | ChIP/ChIP-Seq/Western Blot |
| 使用方法 | Validated Applications: ChIP: 10 µl per ChIP ChIP-Seq & ChIP-chip: 10 µl each WB: 1:500 - 1:1,000 dilution |
| 供应商 | Active Motif |
| 纯化方式 | Affinity Purified |
| 免疫原 | This LXR-β antibody was raised against a peptide in the N-terminal region of human LXR-β. |
| 背景 | LXR-β(Liver X receptor beta, NR1H2, NER, UNR) is an orphan nuclear receptor and a member of the NR1 receptor family. The LXR receptors (including LXR-α and LXR-β) bind oxysterols and play key roles in maintaining cholesterol homeostasis in macrophages, primarily by regulating multiple components of the reverse cholesterol transport. They also are potent inhibitors of inflammation and are capable of repressing cytokine and chemokine production by Toll-like receptor (TLR)-activated macrophages. LXR-β is more widely expressed than LXR-α, particularly in the brain. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified rabbit IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 55 kDa |
| 参考文献 |
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LXR-β pAb tested by ChIP-Chip. ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with chormatin from 2.5 million primary human liver cells. ChIP DNA was amplified by WGA, labeled and hybridized to a human tiling array. The image is zoomed in to show LXR-β binding at a known LXR binding site in the RARB promoter. | |
LXR-β pAb tested by ChIP-qPCR. Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 25 µg of human liver chromatin and 10 µl of LXR-β antibody. ChIP DNA was used in qPCR with the negative control primer pairs and primers against the known LXR binding site in the RARB promoter. Data are presented as Binding Events Detected per 1000 Cells using Active Motifs Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction. | |
LXR-β pAb tested by Western blot. Detection of LXR-β by Western blot analysis. HeLa whole-cell extract (20 µg) was probed with LXR-β pAb at a 1:1000 dilution. |
