| 货号 | 61175 |
| 别名 | NR1H3, LXRA, RLD-1 |
| 同种亚型 | IgG |
| 反应种属 | Human, Mouse |
| 来源宿主 | Rabbit |
| 应用 | ChIP/ChIP-Seq/Western Blot |
| 使用方法 | Validated Applications: ChIP: 10 µl per ChIP ChIP-Seq & ChIP-chip: 10 µl each WB: 1:500 - 1:1,000 dilution |
| 供应商 | Active Motif |
| 纯化方式 | Affinity Purified |
| 免疫原 | This LXR-α antibody was raised against a peptide in the N-terminal region of human LXR-α. |
| 背景 | LXR-α (Liver X receptor alpha, NR1H3) is an orphan nuclear receptor and a member of the NR1 receptor family. The LXR receptors (including LXR-α and LXR-β) bind oxysterols and play key roles in maintaining cholesterol homeostasis in macrophages, primarily by regulating multiple components of the reverse cholesterol transport. They also are potent inhibitors of inflammation and are capable of repressing cytokine and chemokine production by Toll-like receptor (TLR)-activated macrophages. LXR-α is expressed mainly in liver and adipose tissue and interacts with RXR, shifting RXR to an active ligand-binding subunit mediating responses to retinoids. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified rabbit IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 52 kDa |
| 参考文献 |
| 本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。 |
LXR-α antibody (pAb) tested by ChIP-chip. ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with chromatin from 2 million mouse embryonic fibroblasts. ChIP DNA was amplified by WGA, labeled and hybridized to a mouse tiling array. The image is zoomed in to show LXR-α binding at the promoter of the retinoic acid inducible gene Zmiz1. LXR binding at this gene is expected since LXR is known to form heterodimers with RXR (retinoic acid receptor). | |
LXR-α antibody (pAb) tested by ChIP. Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 20 µg of mouse embryonic fibroblast chromatin and 10 µl of LXR-α antibody. ChIP DNA was used in qPCR with the negative control primer pairs or gene-specific primer pairs as indicated. Data are presented as Binding Events Detected per 1000 Cells using Active Motifs Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction. | |
LXR-α antibody (pAb) tested by Western blot. Whole cell extract of Hep G2 cells (30 µg) probed with LXR-α antibody (pAb) at a dilution of 1:500. |
