| 货号 | 61005 |
| 别名 | MEN1, MEAI, SCG2 |
| 同种亚型 | IgG |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | ChIP/ChIP-Seq/Western Blot |
| 使用方法 | Validated Applications: ChIP: 5 µl per ChIP ChIP-Seq & ChIP-chip: 5 µl each WB: 1:500 – 1:1,000 dilution |
| 供应商 | Active Motif |
| 纯化方式 | Affinity Purified |
| 免疫原 | This Menin antibody was raised against a peptide corresponding to amino acids within the C-terminal region of human Menin. |
| 背景 | Menin (MEN1, SCG2) is a tumor suppressor and a component of multiple MLL-containing protein complexes (MLL, ACSOM, MLL2/3 and MLL3/4), which methylate histone H3 at lysine 4. Menin interacts directly with MLL through a domain in the N-terminus of MLL. It also interacts with RNA polymerase II large subunit C-terminal domain phosphorylated at Ser5. Menin is involved in the activation of HOX gene expression and is localized along with MLL at several HOX gene promoters. It is also important for activating the expression of several cell cycle regulators, indicating a mechanism for its role as a tumor suppressor. Defects in Menin are associated with inherited Multiple Endocrine Neoplasia type I, a dominant predisposition to cancer of a number of endocrine tissues. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 80 kDa |
| 参考文献 |
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Menin antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 25 µg of chromatin from a mouse thymus and 5 µg of MEN1 antibody. ChIP DNA was sequenced on the Illumina HiSeq and 15 million sequence tags were mapped to identify MEN1 binding sites. MEN1 is a subunit of the MLL complex which catalyzes H3K4 trimethylation and is therefore expected to localize with H3K4me3. A sampling of the MEN1 ChIP-Seq data shows the expected co-localization of MEN1 and H3K4me3. | |
Western blot of Menin antibody. Nuclear extract of HEK293 cells (20 μg) probed with Menin antibody (1:1,000). |
