| 货号 | 39901 |
| 别名 | WVS, ENX1, EZH1, KMT6, WVS2, ENX-1, EZH2b, KMT6A |
| 同种亚型 | Serum |
| 反应种属 | Human, Mouse |
| 来源宿主 | Rabbit |
| 应用 | ChIP/ChIP-Seq/Immunoprecipitation |
| 使用方法 | Validated Applications: ChIP: 5 µl per ChIP ChIP-Seq: 5 µl each This antibody has not been validated in WB, but a WB-validated antibody is available for this target: 39933. Published Applications: ChIP ChISeq IP See references for more information. Individual optimization may be required. |
| 供应商 | Active Motif |
| 纯化方式 | None |
| 免疫原 | This EZH2 antibody was raised against a recombinant fusion protein corresponding to amino acids 1-370 of mouse EZH2. |
| 背景 | EZH2–(Enhancer of Zeste homolog 2) is a human homolog of the Drosophila Polycomb-group protein Enhancer of Zeste protein. It contains a SET domain that catalyzes the methylation of histone H3 at lysine 27 (Histone H3 Lys27 monomethylated, dimethylated and trimethylated). Polycomb-group proteins repress gene expression by binding to chromatin and locally altering chromatin structure. EZH2, BMI-1 and Suz12 are present in the PRC2 and PRC3 protein complexes that function as mediators of epigenetic transcriptional silencing. EZH2 associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. EZH2 may play a role in the hematopoietic and central nervous systems. Deregulation of EZH2 is linked to a variety of cancers. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, an IgG version (Cat |
| 计算分子量 | 96 kDa |
| 参考文献 |
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EZH2 antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with chromatin from 4 million SUDHL6 B cell lymphoma cells and 5 µl of EZH2 antibody. ChIP DNA was sequenced on the Illumina HiSeq and 25 million sequence tags were mapped to identify EZH2 binding sites. The image on the left shows EZH2 binding at the 5’ end of the CCND2 gene. The image on the right shows EZH2 binding across the repressed HOX gene cluster. | |
EZH2 antibody (pAb) tested by ChIP. ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with chromatin from 4 million SUDHL6 B cell lymphoma cells and 5 µl of EZH2 antibody. ChIP DNA was used in qPCR with the negative control primer pair or gene-specific primer pairs as indicated. Data are presented as Binding Events Detected per 1000 Cells using Active Motifs Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction. |
