| 货号 | 39851 |
| 别名 | CT27, BORIS, CTCF-T, HMGB1L1 |
| 同种亚型 | IgG |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | ChIP/ChIP-Seq/Western Blot |
| 使用方法 | Validated Applications: ChIP: 5 µl per ChIP ChIP-Seq: 5 µl each WB: 1:500 - 1:2,000 dilution ChIP-Seq validation was performed by Active Motifs Epigenetics Services and the complete data set is available in the UCSC Genome Browser by clicking here. |
| 供应商 | Active Motif |
| 纯化方式 | Affinity Purified |
| 免疫原 | This CTBP antibody was raised against a peptide within the N-terminal region of human BORIS. |
| 背景 | BORIS(Brother of Regulator of Imprinted Sites) / CTCFL (CTCF-like) is a zinc-finger DNA binding protein that acts as a chromatin insulator and a paralog of the CTCF protein. Normally, BORIS/CTCFL is expressed only in primary spermatocytes and is thought to play a role in resetting DNA methylation marks during the differentiation of male germ cells. BORIS / CTCFL is also expressed in many cancers and may serve a pro-proliferative function. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 96 kDa |
| 参考文献 |
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Boris / CTCFL antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 30 ug of chromatin from human testis and 2 µl of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 20 million sequence tags were mapped to identify Boris / CTCFL binding sites. The image shows binding across a region of chromosome 7. You can view the complete data set in the UCSC Genome Browser, starting at this specific location, here. | |
BORIS / CTCFL antibody (pAb) tested by Western blot. Whole cell extract (10 μg per lane) from HEK293T cells transfected with either vector alone (lanes 1 and 3), with a FLAG-tagged human BORIS expression vector (lanes 2 and 4) or mouse testis nuclear extract (30 μg, lane 5) was probed with either BORIS / CTCFL antibody at 1:2,000 (lanes 1 and 2) or 1:500 (lane 5) or anti-FLAG tag (lanes 3 and 4). |
