| 货号 | 39781 |
| 别名 | AR, KD, AIS, TFM, DHTR, SBMA, HYSP1, NR3C4, SMAX1, HUMARA |
| 同种亚型 | IgG |
| 反应种属 | Human, Mouse |
| 来源宿主 | Rabbit |
| 应用 | ChIP/ChIP-Seq/Western Blot |
| 使用方法 | Validated Applications: ChIP: 5 µg per ChIP ChIP-Seq: 5 µg each WB: 1 - 2 µg/ml dilution |
| 供应商 | Active Motif |
| 纯化方式 | Protein A Chromatography |
| 免疫原 | This Androgen Receptor antibody was raised against a peptide in the N-terminal region of human Androgen receptor. |
| 背景 | Androgen Receptor (AR, NR3C4) is a nuclear receptor that facilitates signaling of testosterone and other androgenic hormones in the cytoplasm and translocates into the nucleus upon hormone binding. AR serves as a DNA binding activator of transcription of hormone responsive genes. There are two forms of the Androgen Receptor, the AR-A form, lacking the N-terminal 187 amino acids and the full length AR-B form. Androgen Receptor is involved in the development of both primary and secondary male sexual characteristics and abnormal expression of AR is linked to prostate cancer. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | PBS pH 7.5 containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 110 kDa |
| 参考文献 |
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Androgen Receptor antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with chromatin from a human prostate cancer cell line (3 million cells). ChIP DNA was sequenced on the Illumina GAII and 22 million sequence tags were mapped to identify Androgen Receptor binding sites. The image shows hundreds of strong binding sites dispersed across the right arm of human chromosome 16. | |
Androgen Receptor antibody (pAb) tested by ChIP. Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 30 µg of VCAP60 cell chromatin and 10 µg of Androgen receptor antibody. ChIP DNA was used in qPCR with the negative control primer pairs or gene-specific primer pairs as indicated. Data are presented as Binding Events Detected per 1000 Cells using Active Motifs Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction. | |
Androgen Receptor antibody (pAb) tested by Western blot. Nuclear extract (20 µg) of LnCaP (Lane 1) and DU145 cells (Lane 2) probed with Androgen Receptor antibody (pAb) (1 µg/ml). |
