| 货号 | 39369 |
| 别名 | RELA, p65, NFKB3 |
| 同种亚型 | Serum |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | ChIP/Western Blot |
| 使用方法 | Validated Applications: ChIP: 4 µl per ChIP WB: 1:2,500 - 1:5,000 dilution Published Applications: see references |
| 供应商 | Active Motif |
| 纯化方式 | None |
| 免疫原 | This NFkB p65 antibody was raised against a peptide immunogen corresponding to the C-terminal of human p65. |
| 背景 | NFκBp65(Nuclear factorNF-kappa-B p65subunit also known as NFκB p65orRelA) is a member of the Rel family of transcription factors that are involved in processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. The Rel family includes c-Rel, RelA (NFκB p65, NFκB3) and RelB (I-Rel). Rel proteins form dimers with the NFκB p50 (proteolytically processed from p105/NFκB1)and p52 (proteolytically processed from p100/NFκB2) members of the NFκB family that bind DNA and activate transcription. The NFκB p65/p50 heterodimer is the most abundant form of NFκB. NFκB signaling is negatively regulated by the sequestration of the NFκB complex in the cytoplasm by its association with the IκB family of inhibitory proteins. The IκB Kinase (IKK) complex is the key enzyme involved in the activation and translocation of NFκB. The IKK complex is composed of two catalytic subunits (IKKα and IKKβ) and a regulatory subunit (IKKγ). Upon its activation, the IKK complex phosphorylates IκB proteins (IκBα, IκBα phospho Ser32, 36), which marks them for ubiquitination and degradation. This enables the NFκB complex to translocate to the nucleus where it regulates transcription by binding to DNA. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 65 kDa |
| 参考文献 |
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NFκB p65 (pAb) tested by ChIP. ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 2 million HT29 cells treated with TNF and 10 µl of p65 antibody. ChIP DNA was used in qPCR with the negative control primer pair or gene-specific primer pairs as indicated. Data are presented as Binding Events Detected per 1000 Cells using Active Motifs Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction. | |
NFκB p65 (pAb) tested by Western blot. NFκB p65 detection by Western blot. The analysis was performed using 20 µg HEK293 cytoplasmic extract and NFκB p65 pAb at a 1:2,500 dilution. |
