| 货号 | 39058 |
| 别名 | PSG1, B1G1, PBG1, CD66f, PSBG1, PSG95, PSGGA, DHFRP2, PSBG-1, PSGIIA, FL-NCA-1/2, PS-beta-C/D, PS-beta-G-1 |
| 同种亚型 | IgG |
| 反应种属 | Human, Mouse, Rat |
| 来源宿主 | Rabbit |
| 应用 | ChIP/Western Blot |
| 使用方法 | Validated Applications: ChIP: 5 - 10 µl per ChIP WB: 1:500 - 1:1,000 dilution Published Applications: ChIP See references for more information. Individual optimization may be required. For optimal results in Western blotting, primary antibody incubations should be performed at 37°C. Individual optimization may be required. |
| 供应商 | Active Motif |
| 纯化方式 | Affinity Purified |
| 免疫原 | This Sp1 antibody was raised against a synthetic peptide corresponding to amino acid residues 520-534 of human Sp1. |
| 背景 | Sp1(specificity protein 1) is a human transcription factor involved in gene expression in the early development of an organism. The protein is 785 amino acids long, with a molecular weight of 81 kDa. The SP1 transcription factor contains a zinc finger protein motif, by which it binds directly to DNA and enhances gene transcription. Its zinc fingers are of the Cys2/His2 type and bind the consensus sequence 5′-(G/T)GGGCGG(G/A)(G/A)(C/T)-3′ (GC box element). It was discovered in 1983 and has since been modified to form Sp1C, which has a zinc finger protein DNA binding domain. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | PBS containing 0.2% gelatin, 0.01% thimersol and 0.1% sodium azide. Thimersol and sodium azide are highly toxic. |
| 计算分子量 | 97 kDa |
| 参考文献 |
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Sp1 antibody tested by ChIP. Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 20 µg of chromatin from B cell lymphoma Namalwa cells and 6 µl of Sp1 antibody. ChIP DNA was used in qPCR with the control primer pairs or gene-specific primer pairs as indicated. Data are presented as Binding Events Detected per 1000 Cells using Active Motifs Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction. | |
Sp1 antibody tested by Western blot. Detection of Sp1 by Western blot analysis. Sp1 was detected in nuclear extracts derived from K-562 cells using Sp1 rabbit polyclonal antibody at a 1:1,000 dilution. |
