| 货号 | 39099 |
| 别名 | ASH2, Bre2, ASH2L1, ASH2L2 |
| 同种亚型 | Serum |
| 反应种属 | Human, Other (Wide Range) |
| 来源宿主 | Rabbit |
| 应用 | ChIP/ChIP-Seq/Western Blot |
| 使用方法 | Validated Applications: ChIP: 10 µl per ChIP ChIP-Seq: 10 µl each WB: 1:500 - 1:2,000 dilution ChIP-Seq validation was performed by Active Motifs Epigenetics Services and the complete data set is available in the UCSC Genome Browser by clicking here. Published Applications: WB See references for more information. Individual optimization may be required. |
| 供应商 | Active Motif |
| 纯化方式 | None |
| 免疫原 | This ASH2L antibody was raised against full-length recombinant human ASH2 protein. |
| 背景 | ASH2L (absent, small or homeotic discs 2) is a trithorax protein and is a component of different protein complexes, including i.e. mixed-lineage leukemia (MLL) oncoproteins, which contain histone methyltransferase activity. These complexes trimethylate the lysine 4 of histone H3. Histone H3 trimethyl Lys4 is one of histone modifications that are part of elaborate mechanisms that evolved to control the structure of the chromatin. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 77 kDa |
| 参考文献 |
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ASH2L antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 30 ug of chromatin from undifferentiated hESC cells and 10 µl of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 20 million sequence tags were mapped to identify ASH2L binding sites. The image shows binding across a region of chromosome 12. You can view the complete data set in the UCSC Genome Browser, starting at this specific location, here. | |
ASH2L pAb tested by Western blot. ASH2L detection by Western blot. The analysis of ASH2L was performed using HeLa nuclear extract and ASH2L pAb at a 1:2,000 dilution. |
