| 货号 | 61665 |
| 克隆类别 | 1A12G10 |
| 别名 | RPB1, RPO2, POLR2, POLRA, RPBh1, RPOL2, RpIILS, hsRPB1, hRPB220 |
| 同种亚型 | IgG1k |
| 反应种属 | Human |
| 来源宿主 | Mouse |
| 应用 | ChIP/ChIP-Seq/Western Blot/Immunofluorescence/Immunocytochemistry |
| 使用方法 | Validated Applications: ChIP: 5 - 10 µg per ChIP ChIP-Seq: 5 - 10 µg each ICC/IF: 5 µg/ml dilution WB: 0.5 - 2 µl/ml dilution ChIP-Seq validation was performed by Active Motifs Epigenetics Services and the complete data set is available in the UCSC Genome Browser by clicking here. |
| 供应商 | Active Motif |
| 纯化方式 | Protein A Chromatography |
| 免疫原 | This antibody was raised against a synthetic peptide containing phospho-serine 2 and phospho-serine 5 of the CTD of human RNA Polymerase II. |
| 背景 | RNA pol II(RNA polymerase II) is responsible for synthesizing messenger RNA in eukaryotes. RNA pol II contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, RNA pol II, in combination with several other polymerase subunits, form the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. During the transcription cycle, the CTD of the large subunit of RNA pol II is reversibly phosphorylated. RNA pol II containing unphosphorylated CTD is recruited to the promoter, whereas the hyperphosphorylated CTD form is involved in active transcription. Phosphorylation occurs at two sites within the heptapeptide repeat, at serine 2, serine 5 and serine 7. RNA pol II Serine 2 phosphorylation is enriched over the gene body and is associated with transcriptional elongation. RNA pol II Serine 5 phosphorylation is confined to promoter regions and is necessary for the initiation of transcription. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 240 kDa |
| 参考文献 |
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RNA pol II CTD Ser2ph / Ser5ph antibody (mAb) (Clone 1A12G10) tested by ChIP-Seq. ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 30 µg of chromatin from HL-60 cells and 4 µg of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 11 million sequence tags were mapped to identify RNA pol II CTD Ser2ph / Ser5ph binding sites. The image shows binding across a region of chromosome 15. You can view the complete data set in the UCSC Genome Browser, starting at this specific location, here. | |
RNA pol II CTD Ser2ph / Ser5ph antibody (mAb) (Clone 1A12G10) tested by immunofluorescence. Left: HeLa cell stained with RNA pol II CTD Ser2ph / Ser5ph antibody (mAb). Middle:Hoechst. Right: Merge. | |
RNA pol II CTD Ser2ph / Ser5ph antibody (mAb) (Clone 1A12G10) tested by Western blot. HeLa nuclear extract (30 µg per lane) probed with RNA pol II CTD Ser2ph / Ser5ph antibody (mAb) at a 1 µg/ml dilution. |
