| 货号 | 61203 |
| 克隆类别 | 41D |
| 别名 | HEED, WAIT1 |
| 同种亚型 | IgG2a |
| 反应种属 | Human |
| 来源宿主 | Mouse |
| 应用 | ChIP/ChIP-Seq/Immunohistochemistry |
| 使用方法 | Validated Applications: ChIP: 4 µl per ChIP ChIP-Seq & ChIP-chip: 4 µl each IHC: 2 - 8 µg/ml dilution |
| 供应商 | Active Motif |
| 纯化方式 | Protein A Chromatography |
| 免疫原 | This EED antibody was raised against full-length recombinant human EED protein. |
| 背景 | EED (extraembryonic ectoderm) is a component of the Polycomb group (PcG) multiprotein complex PRC2. The PRC2 complex also contains EZH2 and SUZ12 and is recruited to Polycomb response elements and results in the methylation of histone H3 and lysine 27 and the silencing of gene expression. PRC2 initiates repression of the Hox genes during development and is involved in the maintenance of stem cell pluripotency.EED is a member of the superfamily of WD-40 repeat protein family and is required for PRC2-mediated H3K27 methylation. The PRC2 complex may also recruit platform DNMTs, thereby linking two systems of epigenetic repression. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 57 kDa |
| 参考文献 |
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EED antibody (mAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with chromatin from a human B cell lymphoma cell line (4.5 million cells) and 4 µl of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 32 million sequence tags were mapped to identify EED binding sites. The image on the left shows EED binding across a 1.6 million bp region on chromosome 6. The image on the right shows EED binding at the STX16 start site. | |
EED mAb tested by Immunohistochemistry . EED detection by Immunohistochemistry. The analysis was performed using human tonsil paraffin section and the EED mAb. |
