| 货号 | 39789 |
| 克隆类别 | C5.D5 |
| 别名 | MI, WS2, CMM8, WS2A, bHLHe32 |
| 同种亚型 | IgG |
| 反应种属 | Human |
| 来源宿主 | Mouse |
| 应用 | ChIP/ChIP-Seq/Western Blot/Immunoprecipitation/Immunofluorescence/Electrophoretic Mobility Shift Assay/Immunocytochemistry |
| 使用方法 | Validated Applications: ChIP: 2.5 - 10 µg per ChIP ChIP-Seq: 4 µg each Published Applications: IP WB ICC/IF EMSA See references for more information. Individual optimization may be required. |
| 供应商 | Active Motif |
| 纯化方式 | Protein G Chromatography |
| 免疫原 | This MITF antibody was raised against an N-terminal fragment of human MITF. |
| 背景 | MITF protein (Microphthalmia-associated Transcription Factor) is a basic helix-loop-helix type transcription factor involved in the differentiation and development of retinal pigment epithelium and melanocytes. It is also responsible for pigment cell-specific transcription of the melanogenesis enzyme genes tyrosinase and tyrosinase related protein 1. For activity MITF needs to heterodimerize with other bHLH proteins such as TFE3, TFEB or TFE. Defects in MITF are the cause of Waardenburg syndrome type 2A and Tietz syndrome. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 52-56 kDa |
| 参考文献 |
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MITF antibody (mAb) tested by ChIP-Seq. (ChIP) was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) and chromatin from a human melanoma cell line (2.5 million cells). ChIP DNA was sequenced on the Illumina GA II and 19 million sequence tags were mapped to identify MITF binding sites across the genome. The image shows hundreds of MITF binding sites across a 60 million bp region on chromosome 9. | |
MITF antibody (mAb) tested by ChIP. Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 30 µg of chromatin from human lung melanoma cell line MALME-3M and 4 µg of MITF antibody. ChIP DNA was used in qPCR with the control primer pairs or gene-specific primer pairs as indicated. Data are presented as Binding Events Detected per 1000 Cells using Active Motifs Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction. |
