| 货号 | 61695 |
| 克隆类别 | 5F9A9 |
| 同种亚型 | IgG2b |
| 反应种属 | Human, Other (Wide Range) |
| 来源宿主 | Mouse |
| 应用 | Western Blot/Immunofluorescence/Immunocytochemistry |
| 使用方法 | Validated Applications: ICC/IF: 2 - 10 µg/ml dilution WB: 0.5 - 2 µg/ml dilution |
| 供应商 | Active Motif |
| 纯化方式 | Protein A Chromatography |
| 免疫原 | This antibody was raised against a peptide corresponding to phospho-serine 28 of human Histone H3. |
| 背景 | Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. Ser10 phosphorylation and Ser28 phosphorylation in the tail of H3 have very similar kinetics. Both phosphorylations occur early in mitosis when chromosomes begin to condense and during premature chromosome condensation induced in S-phase cells. These phosphorylated serines are excellent mitotic markers. In contrast to Ser10 phosphorylation, Ser28 phosphorylation has never been observed in interphase. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 17 kDa |
| 参考文献 |
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Histone H3S28ph antibody (mAb) (Clone 5F9A9) tested by Immunofluorescence. Left: Formaldehyde fixed mitotic DM4 cell stained with Histone H3S28ph antibody (mAb). Right: Hoechst stain. | |
Histone H3S28ph antibody (mAb) (Clone 5F9A9) tested by Western blot. Detection of Histone H3S28ph antibody by Western blot. The analysis was performed using 20 µg of untreated (lane 1) or colcemid treated (lane 2) HeLa nuclear extract with Histone H3S28ph antibody at a 1 µg/ml dilution. |
