| 货号 | 61623 |
| 克隆类别 | 6G8B7 |
| 同种亚型 | IgG2a |
| 反应种属 | Human, Other (Wide Range) |
| 来源宿主 | Rat |
| 应用 | Western Blot/Immunofluorescence/Enzyme-linked Immunosorbent Assay/Immunocytochemistry |
| 使用方法 | Validated Applications: ICC/IF: 0.5 - 2 µg/ml dilution WB: 0.2 - 2 µg/ml dilution ELISA: Individual optimization required. |
| 供应商 | Active Motif |
| 纯化方式 | Protein G Chromatography |
| 免疫原 | This antibody was raised against a peptide corresponding to amino acids 1-19 including phospho-serine 10 of human Histone H3. |
| 背景 | Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. Interestingly,phosphorylation of Ser10 on histone H3 is involved in both transcription and cell division, two events requiring opposite alterations in the degree of chromatin compaction. Ser10 in the tail of histone H3 is strongly phosphorylated early in mitosis when chromosomes begin to condense, and during premature chromosome condensation induced in S-phase cells. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 17 kDa |
| 参考文献 |
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Histone H3S10ph antibody (mAb) (Clone 6G8B7) tested by immunofluorescence. Top: HeLa cells stained with H3S10ph antibody (mAb) at a dilution of 0.2 μg/ml. Bottom: Merge with Hoechst. | |
Histone H3S10ph antibody (mAb) (Clone 6G8B7) tested by Western blot. HeLa nuclear extract (20 µg) probed with Histone H3S10ph antibody (mAb) at a dilution of 2 µg/ml. | |
Histone H3S10ph antibody (mAb) (Clone 6G8B7) specificity tested by Peptide Array analysis. Peptide array analysis was used to confirm the specificity of this antibody for its intended modification. Histone H3S10ph antibody (mAb) was applied to Active Motifs MODified™ Histone Peptide Array (Catalog No. 13001) at a dilution of 0.3 µg/ml. The arrays were scanned with ArrayAnalysis Software 7 and the results plotted. Recognition of the H3 serine 10 peptides by the antibody may be blocked by modifications at arginine 8, lysine 9, threonine 11 or lysine 14. | |
Histone H3S10ph antibody (mAb) (Clone 6G8B7) tested by ELISA. BSA conjugated peptides were probed to show the specificity of the H3S10ph antibody (mAb). The range of antibody dilution used is as shown. |
