| 货号 | 61533 |
| 克隆类别 | MABI 0422 |
| 同种亚型 | IgG1 |
| 反应种属 | Human, Other (Wide Range) |
| 来源宿主 | Mouse |
| 应用 | Western Blot/Dot Blot |
| 使用方法 | Validated Applications: WB: 0.5 - 2 µg/ml dilution |
| 供应商 | Active Motif |
| 纯化方式 | Protein G Chromatography |
| 免疫原 | This antibody was raised against a synthetic peptide containing dimethyl-lysine 20 of human Histone H4. |
| 背景 | Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. Histone 4 lysine 20 (H4K20) can be mono-, di- or trimethylated by different histone methyltransferases such as NSD1 or ASH1. The methylation of this lysine is often associated with transcriptional repression. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 8 kDa |
| 参考文献 |
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Histone H4K20me2 antibody (mAb) tested by Western blot. Nuclear extract (20 µg) of HeLa cells probed with the Histone H4K20me2 antibody (mAb) at 2 µg/ml. | |
Histone H4K20me2 antibody (mAb) specificity tested by peptide array analysis. Peptide array analysis was used to confirm the specificity of this antibody for its intended modification. Histone H4K20me2 antibody (mAb) was applied at a dilution of 0.5 µg/ml to Active Motifs MODified™ Histone Peptide Array (Catalog No. 13001). The arrays were scanned with ArrayAnalysis Software 7 and the results plotted. Specificity data is shown for the most reactive peptides and those related to the immunogen. | |
Histone H4K20me2 antibody (mAb) tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H4K20me2 antibody (mAb) for dimethyl-Lys20 of histone H4. Peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with Histone H4K20me2 antibody (mAb) at 2 ug/ml. The amount of peptide (picomoles) spotted is indicated next to each row. Top panel: Lane 1: unmodified Lys79 peptide Lane 2: monomethyl-Lys79 H4 peptide Lane 3: dimethyl-Lys79 H4 peptide Lane 4: trimethyl-Lys79 H4 peptide Lane 5: monomethyl-Lys20 H4 peptide Lane 6: dimethyl-Lys20 H4 peptide Lane 7: trimethyl-Lys20 H4 peptide Lane 8: acetyl-Lys20 peptide Lane 9: unmodified Lys20 peptide Bottom panel: Lane 1: monomethyl-Lys31 H4 peptide Lane 2: dimethyl-Lys31 H4 peptide Lane 3: trimethyl-Lys31 H4 peptide Lane 4: monomethyl-Lys44 H4 peptide Lane 5: dimethyl-Lys44 H4 peptide Lane 6: trimethyl-Lys44 H4 peptide. |
