| 货号 | 61429 |
| 克隆类别 | 51Ta2H12 |
| 同种亚型 | IgG1k |
| 反应种属 | Human, Other (Wide Range) |
| 来源宿主 | Mouse |
| 应用 | Western Blot/Immunofluorescence/Dot Blot/Immunocytochemistry |
| 使用方法 | Published Applications: ICC/IF WB DB See references for more information. Individual optimization may be required. |
| 供应商 | Active Motif |
| 纯化方式 | Protein G Chromatography |
| 免疫原 | This antibody was raised against a synthetic peptide corresponding to amino acid residues 180-195 of human TAF10. The antibody recognizes H3 doubly modified at K9me2S10ph and/or K27me2S28ph. |
| 背景 | Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. Histone H3 tails can be dimethylated on lysine (Kme2) and also phosphorylated on the adjacent serine (Sph) residue. H3K9me2S10ph and/or H3K28S29ph have been observed to occur during mitosis and may provide a specific chromatin conformation during chromatin condensation for cell division. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in 100mM Tris-HCl (pH 8.0), 150 mM NaCl, 0.1 mM EDTA, 45 mM glycine, 0.05% sodium azide |
| 计算分子量 | 17 kDa |
| 参考文献 |
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